Ca2+ requirement for photosynthetic oxygen evolution of spinach and mangrove photosystem II membrane preparations
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چکیده
منابع مشابه
Reactivation of Photosynthetic Oxygen Evolution in Tris-inactivated Inside-out Photosystem Ii Vesicles from Spinach
Purified inside-out photosystem II vesicles with retained capacity for oxygen evolution and a simple polypeptide composition were found to be more sensitive to washing with tris(hydroxymethyl)aminomethane (Tris) at alkaline pH than right-side out thylakoids. This suggests that the thylakoid membrane limits the penetration of the active unprotonated form of Tris. The inhibition of photosynthetic...
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Vesicles prepared with the French press from membranes of cyanelles of Cyanophora paradoxa retain O(2) evolution activity with rates up to 500 micromoles 2,6-dichlorophenolindophenol reduced per hour per milligram chlorophyll. This activity is immediately lost when the vesicles are transferred from the sucrose-phosphate-citrate preparation buffer into dilute phosphate buffer. Similar preparatio...
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The half-time of O2 release in Chlorella, thylakoids and Photosystem II membranes has generally been found to be 0.9 to 3.0 ms, but recently Plijter et al. (Biochim. Biophys. Acta 935 (1988) 299-311) reported it to be between 30 to 130 ms. This work presents a new method, using a Oark electrode, for measuring O2 release times in leaf discs, thylakoids and Photosystem II membranes. The sample is...
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The capability of different manganese complexes to act as PS II electron donors in D1/D2/cytochrome b559 complexes has been analyzed by measuring actinic light-induced absorption changes at 680 nm (650 nm) and 340 nm, reflecting the photoaccumulation of Pheophytin⁻ (Pheo⁻) and the reduction of NADP⁺, respectively. The data obtained revealed: a) the donor capacity of synthetic binuclear Mn (III)...
متن کاملOrganization of the oxygen-evolution enzyme complex studied by butanol/water phase partitioning of spinach photosystem II particles.
The oxygen-evolving photosystem II particles prepared from spinach chloroplasts with brief sonication and Triton X-100 treatment were subjected to butanol/water phase partitioning. Three peripheral proteins of photosystem II having relative molecular masses of 33,000, 24,000, and 18,000 daltons and a part of the manganese atoms associated with photosystem II were partitioned into the aqueous ph...
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ژورنال
عنوان ژورنال: FEBS Letters
سال: 1985
ISSN: 0014-5793
DOI: 10.1016/0014-5793(85)80630-x